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The delicate-clipped sequences that overhang the breakpoint are collapsed right into a consensus sequence employing a several sequence alignment (MSA) presented while in the seqAn library [27]. Wham applies three filters to your consensus sequences. Breakpoints aren't described in circumstances exactly where consensus sequences are shorter than 10
Wham is created for paired-end Illumina libraries with standard insert dimensions (~300bp-500bp). Wham integrates mate-pair mapping, break up study mapping, comfortable-clipping, option alignment and consensus sequence based mostly evidence to forecast SV breakpoints with single-nucleotide precision. Wham generates a merged pileup (catalog of reads
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